Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors

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Fluorescent Lactic Acid Micro organism and Bifidobacteria as Automobiles of DNA Microbial Biosensors

Administration and quantification of effector molecules comparable to heavy metals, toxins or totally different objective molecules is of good biotechnological, social and monetary curiosity. Microorganisms have regulatory proteins that acknowledge and modify the gene expression inside the presence or absence of these compounds (effector molecules) through binding to gene sequences. The affiliation of these recognizing gene sequences to reporter genes will allow the detection of effector molecules of curiosity with extreme sensitivity.

As quickly as investigators have these two elements-recognizing gene sequences and reporter genes that emit signals-we need an acceptable vehicle to introduce every elements. Proper right here, we suggest lactic acid micro organism (LAB) and bifidobacteria as promising service microorganisms for these molecular biosensors. Utilizing fluorescent proteins along with food-grade vectors and clustered repeatedly interspaced temporary palindromic repeats (CRISPR) are indispensable devices for introducing biosensors into these microorganisms. Utilizing these LAB and bifidobacteria could be of specific curiosity for studying the intestinal setting or totally different superior ecosystems.

The great variety of species tailor-made to many environments, along with the chance of creating use of a variety of protocols for his or her transformation with recognizing gene sequences and reporter genes are considerable advantages. Lastly, an effort must be made to go looking out recognizable gene sequences.

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DNAAF5 Antibody (HRP)

20-abx308300
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNAAF5 Antibody (HRP)

abx308300-100g 100 µg
EUR 362.5

DNAAF5 Antibody (HRP)

abx308300-20g 20 µg
EUR 162.5

DNAAF5 Antibody (HRP)

abx308300-50g 50 µg
EUR 250

DNAAF5 Antibody (FITC)

20-abx308301
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNAAF5 Antibody (FITC)

abx308301-100g 100 µg
EUR 362.5

DNAAF5 Antibody (FITC)

abx308301-20g 20 µg
EUR 162.5

DNAAF5 Antibody (FITC)

abx308301-50g 50 µg
EUR 250

DNAAF5 Antibody (Biotin)

20-abx308302
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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DNAAF5 Antibody (Biotin)

abx308302-100g 100 µg
EUR 362.5

DNAAF5 Antibody (Biotin)

abx308302-20g 20 µg
EUR 162.5

DNAAF5 Antibody (Biotin)

abx308302-50g 50 µg
EUR 250

DNAAF5 Polyclonal Antibody

A58814
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  • 100 µg
  • 20 ul
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  • 100 ul

DNAAF5 Conjugated Antibody

C42981 100ul
EUR 476.4

DNAAF5 Antibody, HRP conjugated

1-CSB-PA771471LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

DNAAF5 Antibody, FITC conjugated

1-CSB-PA771471LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

DNAAF5 Antibody, Biotin conjugated

1-CSB-PA771471LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OAGA06580-100UL - DNAAF5 Antibody

OAGA06580-100UL 100ul
EUR 369

DNAAF5 Rabbit Polyclonal Antibody

E10G02198 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

DNAAF5 Polyclonal Antibody, HRP Conjugated

A58817
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  • 100 µg
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DNAAF5 Polyclonal Antibody, FITC Conjugated

A58816
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  • 100 µg
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DNAAF5 Polyclonal Antibody, Biotin Conjugated

A58815
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  • 100 µg
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  • 100 ul

Dnaaf5 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR513187 2 nmol Ask for price

DNAAF5 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR310343 2 nmol Ask for price

Dnaaf5 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR420710 2 nmol Ask for price

Human Dynein Assembly Factor 5, Axonemal / HEATR2 (DNAAF5) ELISA Kit

abx387761-96tests 96 tests
EUR 1093.2

Dnaaf5 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL708859 5 µg/vial Ask for price

Dnaaf5 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL518008 5 µg/vial Ask for price

DNAAF5 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL304136 5 µg/vial Ask for price

DNAAF1 antibody

10R-6890 100 ul
EUR 709
Description: Mouse monoclonal DNAAF1 antibody

DNAAF1 antibody

10R-6892 100 ul
EUR 709
Description: Mouse monoclonal DNAAF1 antibody

DNAAF1 antibody

10R-6893 100 ul
EUR 709
Description: Mouse monoclonal DNAAF1 antibody

DNAAF1 Antibody

DF14672 100ul
EUR 420

DNAAF1 Antibody

DF14672-100ul 100ul
EUR 280

DNAAF1 Antibody

DF14672-200ul 200ul
EUR 350

DNAAF2 Antibody

E40PV8515 50ul
EUR 395
Description: Available in various conjugation types.

DNAAF3 Antibody

20-abx313748
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  • 100 ug
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DNAAF3 Antibody

1-CSB-PA850845LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

DNAAF4 Antibody

1-CSB-PA855504LA01HU
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  • 100ug
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

DNAAF3 Antibody

abx313748-100g 100 µg
EUR 362.5

DNAAF3 Antibody

abx313748-20g 20 µg
EUR 162.5

DNAAF3 Antibody

abx313748-50g 50 µg
EUR 250

DNAAF5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

TR304136 5 µg/vial Ask for price

Dnaaf5 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vectorr

TR708859 5 µg/vial Ask for price

DNAAF3 Antibody (HRP)

20-abx313749
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  • 100 ug
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DNAAF3 Antibody (HRP)

abx313749-100g 100 µg
EUR 362.5

DNAAF3 Antibody (HRP)

abx313749-20g 20 µg
EUR 162.5

DNAAF3 Antibody (HRP)

abx313749-50g 50 µg
EUR 250

Dnaaf5 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vectorr

TR518008 5 µg/vial Ask for price

DNAAF3 Antibody (FITC)

20-abx313750
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  • 100 ug
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DNAAF3 Antibody (FITC)

abx313750-100g 100 µg
EUR 362.5

DNAAF3 Antibody (FITC)

abx313750-20g 20 µg
EUR 162.5

DNAAF3 Antibody (FITC)

abx313750-50g 50 µg
EUR 250

DNAAF3 Antibody (Biotin)

20-abx313751
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  • 100 ug
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DNAAF3 Antibody (Biotin)

abx313751-100g 100 µg
EUR 362.5

DNAAF3 Antibody (Biotin)

abx313751-20g 20 µg
EUR 162.5

DNAAF3 Antibody (Biotin)

abx313751-50g 50 µg
EUR 250

DNAAF3 Antibody, HRP conjugated

1-CSB-PA850845LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

DNAAF4 Antibody, HRP conjugated

1-CSB-PA855504LB01HU
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  • 100ug
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

DNAAF3 Antibody, FITC conjugated

1-CSB-PA850845LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

DNAAF4 Antibody, FITC conjugated

1-CSB-PA855504LC01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

OACA09659-50UG - DNAAF3 Antibody

OACA09659-50UG 50ug
EUR 179

DNAAF3 Antibody, Biotin conjugated

1-CSB-PA850845LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against DNAAF3. Recognizes DNAAF3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

DNAAF4 Antibody, Biotin conjugated

1-CSB-PA855504LD01HU
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  • 100ug
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Description: A polyclonal antibody against DNAAF4. Recognizes DNAAF4 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OAGA05889-100UL - DNAAF4 Antibody

OAGA05889-100UL 100ul
EUR 369

OACA09659-100UG - DNAAF3 Antibody

OACA09659-100UG 100ug
EUR 319

DNAAF1 Polyclonal Antibody

29897 100ul
EUR 439

DNAAF1 Polyclonal Antibody

29897-100ul 100ul
EUR 302.4

DNAAF1 Polyclonal Antibody

29897-50ul 50ul
EUR 224.4

DNAAF1 Polyclonal Antibody

E916593 100ul
EUR 225
Description: Available in various conjugation types.

DNAAF1 Polyclonal Antibody

E-AB-66867-120uL 120uL
EUR 320
Description: Unconjugated

DNAAF1 Polyclonal Antibody

E-AB-66867-200uL 200uL
EUR 530
Description: Unconjugated

DNAAF1 Polyclonal Antibody

E-AB-66867-60uL 60uL
EUR 200
Description: Unconjugated

DNAAF1 Polyclonal Antibody

E-AB-66867-each each Ask for price
Description: Unconjugated

ARP52548_P050-25UL - DNAAF4 Antibody - middle region

ARP52548_P050-25UL 25ul
EUR 99

ARP75849_P050-25UL - DNAAF1 Antibody - middle region

ARP75849_P050-25UL 25ul
EUR 99

ARP69705_P050-25UL - DNAAF3 Antibody - C-terminal region

ARP69705_P050-25UL 25ul
EUR 99

ARP69706_P050-25UL - DNAAF3 Antibody - C-terminal region

ARP69706_P050-25UL 25ul
EUR 99

ARP52547_P050-25UL - DNAAF4 Antibody - C-terminal region

ARP52547_P050-25UL 25ul
EUR 99

ARP75848_P050-25UL - DNAAF1 Antibody - N-terminal region

ARP75848_P050-25UL 25ul
EUR 99

DNAAF1 Polyclonal Conjugated Antibody

C29897 100ul
EUR 476.4

ARP69705_P050 - DNAAF3 Antibody - C-terminal region (ARP69705_P050)

ARP69705_P050 100ul
EUR 389

ARP69706_P050 - DNAAF3 Antibody - C-terminal region (ARP69706_P050)

ARP69706_P050 100ul
EUR 389

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

20-abx318218
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  • 100 ug
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

20-abx112209
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  • 150 ul
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

abx112209-100l 100 µl
EUR 612.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

abx318218-100g 100 µg
EUR 362.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

abx318218-20g 20 µg
EUR 162.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody

abx318218-50g 50 µg
EUR 250

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (HRP)

20-abx315110
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  • 100 ug
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (FITC)

20-abx315111
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  • 100 ug
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (HRP)

abx315110-100g 100 µg
EUR 362.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (HRP)

abx315110-20g 20 µg
EUR 162.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (HRP)

abx315110-50g 50 µg
EUR 250

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (FITC)

abx315111-100g 100 µg
EUR 362.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (FITC)

abx315111-20g 20 µg
EUR 162.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (FITC)

abx315111-50g 50 µg
EUR 250

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (Biotin)

20-abx315112
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  • 100 ug
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Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (Biotin)

abx315112-100g 100 µg
EUR 362.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (Biotin)

abx315112-20g 20 µg
EUR 162.5

Dyslexia Susceptibility 1 Candidate 1 / DYX1C1 (DNAAF4) Antibody (Biotin)

abx315112-50g 50 µg
EUR 250

Dnaaf1/ Rat Dnaaf1 ELISA Kit

ELI-50643r 96 Tests
EUR 1063.2

Dnaaf2/ Rat Dnaaf2 ELISA Kit

ELI-08767r 96 Tests
EUR 1063.2

Dnaaf3 (untagged) - Rat dynein, axonemal, assembly factor 3 (Dnaaf3)

RN216952 10 µg Ask for price

DNAAF1 siRNA

20-abx914304
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  • 15 nmol
  • 30 nmol

DNAAF1 siRNA

20-abx914305
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  • 15 nmol
  • 30 nmol

DNAAF2 siRNA

20-abx914306
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  • 15 nmol
  • 30 nmol

DNAAF2 siRNA

20-abx914307
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  • 15 nmol
  • 30 nmol

DNAAF3 siRNA

20-abx914308
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  • 15 nmol
  • 30 nmol

DNAAF3 siRNA

20-abx914309
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  • 15 nmol
  • 30 nmol

DNAAF1 siRNA

20-abx901521
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  • 15 nmol
  • 30 nmol

DNAAF2 siRNA

20-abx901522
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  • 15 nmol
  • 30 nmol

DNAAF3 siRNA

20-abx901523
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  • 15 nmol
  • 30 nmol

Dnaaf3 (myc-DDK-tagged) - Rat dynein, axonemal, assembly factor 3 (Dnaaf3)

RR216952 10 µg Ask for price

DNAAF1 Rabbit pAb

A16593-100ul 100 ul
EUR 369.6

DNAAF1 Rabbit pAb

A16593-200ul 200 ul
EUR 550.8

DNAAF1 Rabbit pAb

A16593-20ul 20 ul
EUR 219.6

DNAAF1 Rabbit pAb

A16593-50ul 50 ul
EUR 267.6

DNAAF1 ELISA KIT|Human

EF004885 96 Tests
EUR 826.8

Dnaaf1 ORF Vector (Rat) (pORF)

ORF066087 1.0 ug DNA
EUR 607.2

Dnaaf2 ORF Vector (Rat) (pORF)

ORF066088 1.0 ug DNA
EUR 607.2

Rat DNAAF1 shRNA Plasmid

20-abx990192
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  • 150 µg
  • 300 µg

Rat DNAAF2 shRNA Plasmid

20-abx990437
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  • 150 µg
  • 300 µg

Rat DNAAF3 shRNA Plasmid

20-abx991254
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  • 150 µg
  • 300 µg

DNAAF1 ORF Vector (Human) (pORF)

ORF018393 1.0 ug DNA Ask for price

DNAAF2 ORF Vector (Human) (pORF)

ORF018394 1.0 ug DNA Ask for price

DNAAF3 ORF Vector (Human) (pORF)

ORF018395 1.0 ug DNA Ask for price

Dnaaf1 3'UTR GFP Stable Cell Line

TU253484 1.0 ml Ask for price

Dnaaf2 3'UTR GFP Stable Cell Line

TU253485 1.0 ml Ask for price

DNAAF3 (GFP-tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 4

RG234249 10 µg Ask for price

DNAAF3 (GFP-tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 3

RG234393 10 µg Ask for price

DNAAF3 (GFP-tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 1

RG234555 10 µg Ask for price

DNAAF3 (untagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 1

SC332446 10 µg Ask for price

DNAAF3 (untagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 3

SC332447 10 µg Ask for price

DNAAF3 (untagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 4

SC332448 10 µg Ask for price

Human DNAAF2 shRNA Plasmid

20-abx960524
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  • 150 µg
  • 300 µg

Human DNAAF1 shRNA Plasmid

20-abx964742
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  • 150 µg
  • 300 µg

Mouse DNAAF3 shRNA Plasmid

20-abx984074
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  • 150 µg
  • 300 µg

Human DNAAF3 shRNA Plasmid

20-abx967554
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  • 150 µg
  • 300 µg

Mouse DNAAF2 shRNA Plasmid

20-abx979964
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  • 150 µg
  • 300 µg

Mouse DNAAF1 shRNA Plasmid

20-abx976562
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  • 150 µg
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DNAAF3 (Myc-DDK tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 4

RC234249 10 µg Ask for price

DNAAF3 (Myc-DDK tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 3

RC234393 10 µg Ask for price

DNAAF3 (Myc-DDK tagged) - Homo sapiens dynein, axonemal, assembly factor 3 (DNAAF3), transcript variant 1

RC234555 10 µg Ask for price

Human DNAAF1 knockout cell line

ABC-KH4268 1 vial Ask for price
Description: Human DNAAF1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human DNAAF2 knockout cell line

ABC-KH4269 1 vial Ask for price
Description: Human DNAAF2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human DNAAF3 knockout cell line

ABC-KH4270 1 vial Ask for price
Description: Human DNAAF3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human DNAAF1 knockdown cell line

ABC-KD4268 1 vial Ask for price
Description: Human DNAAF1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human DNAAF2 knockdown cell line

ABC-KD4269 1 vial Ask for price
Description: Human DNAAF2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human DNAAF3 knockdown cell line

ABC-KD4270 1 vial Ask for price
Description: Human DNAAF3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human DNAAF1 Protein Lysate 20ug

IHUDNAAF1PLLY20UG each
EUR 213
Description: Human DNAAF1 Protein Lysate 20ug

Human DNAAF2 Protein Lysate 20ug

IHUDNAAF2PLLY20UG each
EUR 361
Description: Human DNAAF2 Protein Lysate 20ug

Human DNAAF3 Protein Lysate 20ug

IHUDNAAF3PLLY20UG each
EUR 361
Description: Human DNAAF3 Protein Lysate 20ug

Human DNAAF4 Protein Lysate 20ug

IHUDNAAF4PLLY20UG each
EUR 213
Description: Human DNAAF4 Protein Lysate 20ug

Dnaaf1 Protein Vector (Rat) (pPM-C-HA)

PV264348 500 ng
EUR 723.6

Dnaaf2 Protein Vector (Rat) (pPM-C-HA)

PV264352 500 ng
EUR 1399.2

Dnaaf1 Protein Vector (Rat) (pPB-C-His)

PV264346 500 ng
EUR 723.6

Dnaaf1 Protein Vector (Rat) (pPB-N-His)

PV264347 500 ng
EUR 723.6

Dnaaf1 Protein Vector (Rat) (pPM-C-His)

PV264349 500 ng
EUR 723.6

Dnaaf2 Protein Vector (Rat) (pPB-C-His)

PV264350 500 ng
EUR 1399.2

Irregular Homocysteine Metabolism: An Notion of Alzheimer’s Sickness from DNA Methylation

Alzheimer’s sickness (AD) is a persistent neurodegenerative sickness inside the central nervous system that has superior pathogenesis inside the aged. The current analysis focuses on the epigenetic mechanisms of AD, in response to the most recent findings. The best-characterized chromatin modifications in epigenetic mechanisms is DNA methylation. Extraordinarily replicable data reveals that AD incidence is often accompanied by methylation diploma changes of the AD-related gene.

Homocysteine (Hcy) is simply not solely an intermediate product of one-carbon metabolism however as well as an important neutral hazard concern of AD; it could probably affect the cognitive carry out of the thoughts by altering the one-carbon metabolism and interfering with the DNA methylation course of, resulting in cerebrovascular sickness.

Often, Hcy may be an environmental concern that impacts AD by means of the DNA methylation pathway with a group of changes in AD-related substance. This analysis will take into consideration the relation between DNA methylation and Hcy and try to find out their rule inside the pathophysiology of AD.

TET is focused for proteasomal degradation by the PHD-pVHL pathway to scale back DNA hydroxymethylation

Hypoxia-inducible elements are heterodimeric transcription elements that play an necessary place in a cell’s functionality to adapt to low oxygen. The von-Hippel Lindau tumor suppressor (pVHL), acts as a grasp regulator of HIF train, and its specializing in of prolyl hydroxylated HIF-α for proteasomal degradation beneath normoxia is taken into account a big mechanism for pVHL tumor suppression and cellular response to oxygen.

Whether or not or not pVHL regulates totally different targets by the identical mechanism is actually unknown. Proper right here, we decide TET2/Three as novel targets of pVHL. pVHL induces proteasomal degradation of TET2/3, resulting in decreased worldwide 5-hydroxymethylcytosine ranges.

Conserved proline residues contained in the LAP/LAP-like motifs of these two proteins are hydroxylated by the prolyl hydroxylase enzymes (PHD2/EGLN1 and PHD3/EGLN3), which is prerequisite for pVHL-mediated degradation. Using zebrafish as a model, we determined that worldwide 5-hydroxymethylcytosine ranges are enhanced in vhl-null, egln1a/b-double null and egln3-null embryos.

Subsequently, we reveal a novel carry out for the PHD-pVHL pathway in regulating TET protein stability and train. These data lengthen our understanding of how TET proteins are regulated and provide new notion into the mechanisms of pVHL in tumor suppression.

Degree-of-care DNA testing by routinely and sequentially performing extraction, amplification and identification in a closed-type cassette

  • Nucleic acid detection is important for scientific diagnostics; nonetheless, it is tough to hold out genetic testing on the point-of-care due to the tedious steps involved in DNA extraction and the prospect of cross-contamination from amplicons.
  • To comprehend a fully-automated and contamination-free nucleic acid detection, we propose a closed-type cassette system which permits the following steps to be operated routinely and sequentially: sample preparation primarily based totally on magnetic beads, objective amplification using multiplex polymerase chain response, and colorimetric detection of amplicons using a serial invasive response coupled with the aggregation of gold nanoparticle probes.
  • The cassette was designed to be spherical and closed, and 10 targets in a sample is perhaps concurrently detected by the naked eye or using a spectrophotometer inside the system.
  • In addition to, a cassette-driven system was fabricated to modify reagents between wells, to manage the temperature of each response, and to sense the colour inside the detection wells. The cassette system was delicate adequate to detect 10 genotypes at 5 single nucleotide polymorphism web sites related to the anticoagulant’s utilization, via using a 0.5 µL blood sample.
  • The accuracy of the system was evaluated by detecting 12 total blood samples, and the outcomes obtained have been in line with these obtained using pyrosequencing. The cassette is airtight and the complete system is totally computerized; the one information operation is the addition of the sample to the cassette, performing point-of-care genetic testing in a sample-in/answer-out means.

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