Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors
Greg
May 24, 2021
Fluorescent Lactic Acid Micro organism and Bifidobacteria as Automobiles of DNA Microbial Biosensors
Administration and quantification of effector molecules comparable to heavy metals, toxins or totally different objective molecules is of good biotechnological, social and monetary curiosity. Microorganisms have regulatory proteins that acknowledge and modify the gene expression inside the presence or absence of these compounds (effector molecules) through binding to gene sequences. The affiliation of these recognizing gene sequences to reporter genes will allow the detection of effector molecules of curiosity with extreme sensitivity.
As quickly as investigators have these two elements-recognizing gene sequences and reporter genes that emit signals-we need an acceptable vehicle to introduce every elements. Proper right here, we suggest lactic acid micro organism (LAB) and bifidobacteria as promising service microorganisms for these molecular biosensors. Utilizing fluorescent proteins along with food-grade vectors and clustered repeatedly interspaced temporary palindromic repeats (CRISPR) are indispensable devices for introducing biosensors into these microorganisms. Utilizing these LAB and bifidobacteria could be of specific curiosity for studying the intestinal setting or totally different superior ecosystems.
The great variety of species tailor-made to many environments, along with the chance of creating use of a variety of protocols for his or her transformation with recognizing gene sequences and reporter genes are considerable advantages. Lastly, an effort must be made to go looking out recognizable gene sequences.

phylowidget
DNAAF5 Antibody, HRP conjugated |
1-CSB-PA771471LB01HU |
Cusabio |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
DNAAF5 Antibody, FITC conjugated |
1-CSB-PA771471LC01HU |
Cusabio |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
DNAAF5 Antibody, Biotin conjugated |
1-CSB-PA771471LD01HU |
Cusabio |
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Description: A polyclonal antibody against DNAAF5. Recognizes DNAAF5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
DNAAF5 Polyclonal Antibody, Biotin Conjugated |
A58815 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: Ask the seller for details |
DNAAF5 Polyclonal Antibody, FITC Conjugated |
A58816 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: The best epigenetics products |
DNAAF5 Polyclonal Antibody, HRP Conjugated |
A58817 |
EpiGentek |
100 µg |
EUR 570.55 |
Description: kits suitable for this type of research |
Human Dynein Assembly Factor 5, Axonemal / HEATR2 (DNAAF5) ELISA Kit |
abx387761-96tests |
Abbexa |
96 tests |
EUR 911 |
|
H2B Antibody Antibody |
AF4659 |
Affbiotech |
200ul |
EUR 376 |
Description: H2B Antibody Antibody detects endogenous levels of H2B. |
anti- Antibody^Polyclonal antibody control antibody |
LSMab09882 |
Lifescience Market |
100 ug |
EUR 438 |
Ly1 Antibody Reactive (LYAR) Antibody |
20-abx123734 |
Abbexa |
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Anti-Glycolipid Antibody (AGA) Antibody |
abx036399-100ug |
Abbexa |
100 ug |
EUR 391 |
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Ly1 Antibody Reactive (LYAR) Antibody |
20-abx014333 |
Abbexa |
-
EUR 314.00
-
EUR 98.00
-
EUR 398.00
-
EUR 495.00
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
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Ly1 Antibody Reactive (LYAR) Antibody |
20-abx008109 |
Abbexa |
-
EUR 300.00
-
EUR 439.00
-
EUR 189.00
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Anti-Glycolipid Antibody (AGA) Antibody |
20-abx004855 |
Abbexa |
-
EUR 411.00
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EUR 592.00
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EUR 182.00
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EUR 314.00
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- 100 ul
- 200 ul
- 20 ul
- 50 ul
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Ly1 Antibody Reactive (LYAR) Antibody |
abx033330-400ul |
Abbexa |
400 ul |
EUR 523 |
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Ly1 Antibody Reactive (LYAR) Antibody |
abx033330-80l |
Abbexa |
80 µl |
EUR 286 |
|
Anti-Glycoprotein Antibody (GP) Antibody |
20-abx319900 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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Anti-Glycoprotein Antibody (GP) Antibody |
20-abx319901 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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Anti-Glycoprotein Antibody (GP) Antibody |
20-abx319905 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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Anti-Glycoprotein Antibody (GP) Antibody |
20-abx319913 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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Ly1 Antibody Reactive (LYAR) Antibody |
20-abx311665 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
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- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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Anti-Glycolipid Antibody (AGA) Antibody |
abx230204-100ug |
Abbexa |
100 ug |
EUR 481 |
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Ly1 Antibody Reactive (LYAR) Antibody |
abx234901-100ug |
Abbexa |
100 ug |
EUR 551 |
|
Anti-Anti-SEPT6 antibody antibody |
STJ11100949 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
Anti-Anti-SEPT9 Antibody antibody |
STJ111369 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
Anti-Anti-SEPT4 Antibody antibody |
STJ112276 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
Anti-Anti-SEPT5 Antibody antibody |
STJ25477 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-SEPT8 Antibody antibody |
STJ25479 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT7 Antibody antibody |
STJ28963 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Ly1 Antibody Reactive (LYAR) Antibody |
20-abx324434 |
Abbexa |
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Anti-Anti-SEPT7 Antibody antibody |
STJ116214 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT8 Antibody antibody |
STJ117206 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT12 Antibody antibody |
STJ117759 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT1 antibody antibody |
STJ119580 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
Anti-Anti-SEPT5 Antibody antibody |
STJ114819 |
St John's Laboratory |
100 µl |
EUR 277 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Ly1 Antibody Reactive Homolog (LYAR) Antibody |
20-abx103034 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1302.00
-
EUR 620.00
-
EUR 342.00
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- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
20-abx103035 |
Abbexa |
-
EUR 467.00
-
EUR 133.00
-
EUR 1344.00
-
EUR 634.00
-
EUR 342.00
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- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
20-abx103036 |
Abbexa |
-
EUR 481.00
-
EUR 133.00
-
EUR 1414.00
-
EUR 662.00
-
EUR 356.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
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Hepatitis C Virus Antibody (HCV) Antibody |
abx023924-1mg |
Abbexa |
1 mg |
EUR 1205 |
|
Anti Deoxyribonucleic Acid Antibody (DNA) Antibody |
abx411057-50ug |
Abbexa |
50 ug |
EUR 592 |
|
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
20-abx319902 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
20-abx319903 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
20-abx319904 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
20-abx319906 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
20-abx319907 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
20-abx319908 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
20-abx319914 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
20-abx319915 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
20-abx319916 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
20-abx319929 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
20-abx319930 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
20-abx319931 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Ly1 Antibody Reactive (LYAR) Antibody (HRP) |
20-abx311666 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Ly1 Antibody Reactive (LYAR) Antibody (FITC) |
20-abx311667 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Ly1 Antibody Reactive (LYAR) Antibody (Biotin) |
20-abx311668 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Anti-Glycoprotein 210 Antibody (gp210) Antibody |
abx233571-100ug |
Abbexa |
100 ug |
EUR 481 |
|
Antibody for Human alpha Tubulin Antibody |
SPC-692D |
Stressmarq |
0.1mg |
EUR 354 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is unconjugated. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A390 |
Stressmarq |
0.1mg |
EUR 401 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 390. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A488 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 488. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A565 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 565. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A594 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 594. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A633 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 633. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A655 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 655. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A680 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 680. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-A700 |
Stressmarq |
0.1mg |
EUR 400 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 700. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-ALP |
Stressmarq |
0.1mg |
EUR 394 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Alkaline Phosphatase. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-APC |
Stressmarq |
0.1mg |
EUR 399 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC . |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-APCCY7 |
Stressmarq |
0.1mg |
EUR 471 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC/Cy7. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-BI |
Stressmarq |
0.1mg |
EUR 396 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Biotin. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-DY350 |
Stressmarq |
0.1mg |
EUR 414 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 350. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-DY405 |
Stressmarq |
0.1mg |
EUR 403 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 405. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-DY488 |
Stressmarq |
0.1mg |
EUR 393 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 488. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-DY594 |
Stressmarq |
0.1mg |
EUR 395 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 594. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-DY633 |
Stressmarq |
0.1mg |
EUR 390 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 633. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-FITC |
Stressmarq |
0.1mg |
EUR 392 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to FITC. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-HRP |
Stressmarq |
0.1mg |
EUR 388 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to HRP. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-P594 |
Stressmarq |
0.1mg |
EUR 407 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to PE/ATTO 594. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-PCP |
Stressmarq |
0.1mg |
EUR 399 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to PerCP. |
Antibody for Human alpha Tubulin Antibody |
SPC-692D-RPE |
Stressmarq |
0.1mg |
EUR 397 |
Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to RPE . |
Irregular Homocysteine Metabolism: An Notion of Alzheimer’s Sickness from DNA Methylation
Alzheimer’s sickness (AD) is a persistent neurodegenerative sickness inside the central nervous system that has superior pathogenesis inside the aged. The current analysis focuses on the epigenetic mechanisms of AD, in response to the most recent findings. The best-characterized chromatin modifications in epigenetic mechanisms is DNA methylation. Extraordinarily replicable data reveals that AD incidence is often accompanied by methylation diploma changes of the AD-related gene.
Homocysteine (Hcy) is simply not solely an intermediate product of one-carbon metabolism however as well as an important neutral hazard concern of AD; it could probably affect the cognitive carry out of the thoughts by altering the one-carbon metabolism and interfering with the DNA methylation course of, resulting in cerebrovascular sickness.
Often, Hcy may be an environmental concern that impacts AD by means of the DNA methylation pathway with a group of changes in AD-related substance. This analysis will take into consideration the relation between DNA methylation and Hcy and try to find out their rule inside the pathophysiology of AD.
TET is focused for proteasomal degradation by the PHD-pVHL pathway to scale back DNA hydroxymethylation
Hypoxia-inducible elements are heterodimeric transcription elements that play an necessary place in a cell’s functionality to adapt to low oxygen. The von-Hippel Lindau tumor suppressor (pVHL), acts as a grasp regulator of HIF train, and its specializing in of prolyl hydroxylated HIF-α for proteasomal degradation beneath normoxia is taken into account a big mechanism for pVHL tumor suppression and cellular response to oxygen.
Whether or not or not pVHL regulates totally different targets by the identical mechanism is actually unknown. Proper right here, we decide TET2/Three as novel targets of pVHL. pVHL induces proteasomal degradation of TET2/3, resulting in decreased worldwide 5-hydroxymethylcytosine ranges.
Conserved proline residues contained in the LAP/LAP-like motifs of these two proteins are hydroxylated by the prolyl hydroxylase enzymes (PHD2/EGLN1 and PHD3/EGLN3), which is prerequisite for pVHL-mediated degradation. Using zebrafish as a model, we determined that worldwide 5-hydroxymethylcytosine ranges are enhanced in vhl-null, egln1a/b-double null and egln3-null embryos.
Subsequently, we reveal a novel carry out for the PHD-pVHL pathway in regulating TET protein stability and train. These data lengthen our understanding of how TET proteins are regulated and provide new notion into the mechanisms of pVHL in tumor suppression.
Degree-of-care DNA testing by routinely and sequentially performing extraction, amplification and identification in a closed-type cassette
- Nucleic acid detection is important for scientific diagnostics; nonetheless, it is tough to hold out genetic testing on the point-of-care due to the tedious steps involved in DNA extraction and the prospect of cross-contamination from amplicons.
- To comprehend a fully-automated and contamination-free nucleic acid detection, we propose a closed-type cassette system which permits the following steps to be operated routinely and sequentially: sample preparation primarily based totally on magnetic beads, objective amplification using multiplex polymerase chain response, and colorimetric detection of amplicons using a serial invasive response coupled with the aggregation of gold nanoparticle probes.
- The cassette was designed to be spherical and closed, and 10 targets in a sample is perhaps concurrently detected by the naked eye or using a spectrophotometer inside the system.
- In addition to, a cassette-driven system was fabricated to modify reagents between wells, to manage the temperature of each response, and to sense the colour inside the detection wells. The cassette system was delicate adequate to detect 10 genotypes at 5 single nucleotide polymorphism web sites related to the anticoagulant’s utilization, via using a 0.5 µL blood sample.
- The accuracy of the system was evaluated by detecting 12 total blood samples, and the outcomes obtained have been in line with these obtained using pyrosequencing. The cassette is airtight and the complete system is totally computerized; the one information operation is the addition of the sample to the cassette, performing point-of-care genetic testing in a sample-in/answer-out means.